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KMID : 0545119990090060826
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 6 p.826 ~ p.831
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Comparative Study of Immune-Enhancing Activity of Crude and Mannoprotein-Free Yeast-Glucan Preparations
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Kim, Hye Nam
Lee, Jung Nam/Kim, Gi Eun/Lee, Young Mie Ha/Kim, Chan Wha/Sohn, Jeong Won
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Abstract
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β-Glucan, one of the major cell wall components of Saccharomyces cerevisiae, is known to enhance the immune function, especially by activating macrophages. Accordingly, in an effort to develop a safe and efficient immune stimulatory agent, we prepared crude β-glucan (glucan-p1) and partially purified β-glucan that was free of mannoproteins (glucan-p2), and evaluated their effect on both the macrophage function and resistance to E. coli-induced peritonitis. To investigate the function of the macrophages, phagocytosis, TNF-α secretion, oxygen burst, and the expression of cytokine genes such as IFN-γ and IL-12 were analyzed. Glucan-p2 markedly stimulated the macrophages with all these parameters. Glucan-p1, however, did not stimulate phagocytosis, yet it induced TNF-α secretion, oxygen burst, and the expression of IFN-γ and IL-12, although less efficiently than glucan-p2. Finally, to test the in vivo protective effect of β-glucan against infection, the survival of mice from E. coli-induced peritonitis was investigated. After 24h of the peritoneal challenge of E. coli, all of the mice treated with glucan-p2 survived whereas none survived in the control group. Glucan-p1 showed only a marginal effect in protecting the mice. These results suggest that mannoprotein-free glucan-p2, but not glucan-p1, can serve as an effective immune-stimulating agent.
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